Viscoelasticity measurement comparison between microelastography and surface fluctuations
* Presenting author
Background: Microelastography is a recent technique used to measure viscoelasticity of small objects at the micrometer scale. A recent work has applied the technique on a mouse oocyte, with a micropipette inducing a 15 kHz shear wave, and the oocyte was observed with a 200.000 fps camera through a microscope. However, this technique lacks of gold standard due to the high frequency of vibration. In the other hand, another technique observing surface fluctuations allows to measure sample viscoelasticity at a wide range of frequencies.Aim: The objective was to compare both techniques on a well-defined viscoelastic medium.Methods: A viscoelastic carbopol sample was built. Microelastography used a 50 um diameter pipette to induce the shear wave at various frequency. An high speed camera tracked the shear wave. Elasticity reconstructed with passive elastography. The second technique was built using a 632 nm laser deviated towards the object surface with a prism, and a CCD camera to measure laser deviationResults and conclusions: Viscoelastic measurements were comparable in both methods. Differences are supposed to be due to elasticity variation between surface and bulk of the medium.