DNA amplification by sound and ultrasound frequency vibration
* Presenting author
In the Polymerase Chain Reaction (PCR) method, which is a strong DNA amplification technology, a step with a high temperature of 94 ℃ is inevitably included to denature double-stranded DNA to single-stranded DNA. However, the thermal denaturation has problems causing DNA damage and enzyme deactivation. To overcome them, we have proposed and developed a new technology for DNA denaturation and amplification, namely, vibration-driven DNA amplification method (the frequency ranges from 100 Hz to 25 kHz). In our method, DNA is denatured and amplified by vibrating a micro tube with at sound to ultrasound frequencies using a wave transducer. In the micro tube, DNA, enzyme, and other materials are mixed and dissolved in water. Using the DNA amplification system we developed, we examined the optimum condition for DNA denaturation and amplification by changing amplitude, frequency and time of vibration. Maximum amplification rates were obtained at frequencies from 200 Hz to 500 Hz. The analysis through the electrophoresis with a microchip demonstrated that DNA amplification rate was achieved to 1.7 for a cycle (2 is the ideal value). Moreover, through the agarose gel electrophoresis, we found that no DNA was damaged by vibration.